黑素皮质素受体1（MC1R）基因与VC-獭兔毛色关系的研究

doi:10.11843/j.issn.0366-6964.2013.06.009

Abstract

Abstract:

The study aimed to study the relationship between the melanocortin 1 receptor gene (MC1R) and the coat color in rex-rabbits. According to the sequence of MC1R in GenBank，a pair of specific primer was designed to amplify the cDNA sequence of MC1R of rex-rabbits using RT-PCR technique. The results showed that the 951 bp cDNA sequence encoded 317AA, isoelectric point of MC1R protein was 9.26. Compared with the published sequence in GenBank, there were 7 transmembrane domains. Homology of nucleotide sequence and deduced amino acid was higher than 95% and 93% with cattle, horse, yak, sheep, camel, and so on, which was confirmed by the analysis of phylogenetic tree. The mutation frequency of 14 loci were higher, and 5 amino acid substitutions were detected in rex-rabbits, which lies on the transmembrane domains, which showed that the sequence of MC1R was highly conservative in mammalian. These mutation loci provide the theotrtical basis for studying the relationship between MC1R and coat color in rex-rabbits.

CLC Number:

GAO Rong-kun, CHEN Wei, QU Hai-e, LI Jian-ping, ZHANG Qiao-ling. Study on the Relationship between the Melanocortin 1 Receptor Gene (MC1R) and the Coat Color in Rex-rabbits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 888-893.

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Study on the Relationship between the Melanocortin 1 Receptor Gene (MC1R) and the Coat Color in Rex-rabbits

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